|
KMID : 0624620200530050248
|
|
BMB Reports
2020 Volume.53 No. 5 p.248 ~ p.253
|
|
|
Human transcription factor YY1 could upregulate the HIV-1 gene expression
|
|
Yu Kyung-Lee
Jung Yu-Mi
Park Seong-Hyun
Lee Seong-Deok
You Ji-Chang
|
|
|
Abstract
|
|
|
|
Gene expression in HIV-1 is regulated by the promoters in 5’ long-terminal repeat (LTR) element, which contain multiple DNA regulatory elements that serve as binding sites for cellular transcription factors. YY1 could repress HIV-1 gene expression and latent infection. Here, however, we observed that virus production can be increased by YY1 over-expression and decreased under YY1 depleted condition by siRNA treatment. To identify functional domain(s) of YY1 activation, we constructed a number of YY1 truncated mutants. Our data show that full-length YY1 enhances the viral transcription both through U3 and U3RU5 promoters. Moreover, the C-terminal region (296-414 residues) of YY1 is responsible for the transcriptional upregulation, which could be enhanced further in the presence of the viral Tat protein. The central domain of YY1 (155-295 residues) does not affect LTR activity but has a negative effect on HIV-1 gene expression. Taken together, our study shows that YY1 could act as a transcriptional activator in HIV-1 replication, at least in the early stages of infection.
|
|
|
KEYWORD
|
|
|
HIV-1, Tat, transcription regulation, YY1
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|